AG WAGISTRASSE27A8952SCHLIEREN-ZURICHSWITZERLAND PHONE+41442002000FAX+41442002010 INFO@PRIONICS.COMWWW.PRIONICS.COM EstablishedSalmonellosisdetection.PrioCHECK® SALMONELLAAbPORCINEPRODUCTS RELIABLE,SIMPLE,CONVENIENTANDECONOMICALTESTSFORSMALLANDLARGESCALESCREENINGOFSALMONELLAINFECTIONSINPIGS. WWW.PRIONICS.COMWWW.PRIONICS.COM SALMONELLOSIS SalmonellosisisoneofthemostimportantzoonoticdiseasescausedbyanismsofthetwospeciesofSalmonella(SalmonellaentericaandSalmonellabongori).ThepresenceofSalmonellosishasbeen­identifiedincountriesthroughouttheworld,butappearstobemostprevalentinareasofintensiveanimalhusbandry,especiallyurringinpigsandcalvesandsometypesofpoultry.Thediseasecanaffectalldomesticanimals;howeveryounganimals,pregnantandlactatinganimalsarethemostsusceptibletothedisease.Manyanimals,­especiallypigsandpoultry,mayalsobeinfectedbutshownoclinicalsymptoms.Suchanimalsmaybeimportantinrelationtothespreadofinfectionbetweenflocksandherdsandalsoasasourceoffoodcontaminationandhumaninfections.Inordertocontrolandminimizetheriskofcontamination,controlofthe(pork)supplychainisnecessary. PrionicsoffersaneffectivetoolforthediagnosisofSalmonellainpigs.ThePrioCHECK®SalmonellaAbporcineELISAsarereliable,convenientandeconomicaltestsforsmallandlargescalescreeningofSalmonellainfectionsinpigs. PrioCHECK®SalmonellaAbporcineprocedure color:positive wash Incubationserumsample90minutesRT Incubationconjugate1hourRT wash Detection15minutesRT nocolor:negative PuriedLPSofS.TyphimurimandS.Choleraesuis Positivesample Rabbitanti-swineHRPOconjugate BENEFITS Reliableandproventest ThePrioCHECK®SalmonellaAbporcineismercialversionoftheDanishSalmonellamix-ELISAasdevelopedbytheDanishVeterinaryInstitute(DVI).TheDanishtesthasbeenessfullyandreliablyappliedintheDanishSalmonellacontrolprogramandisconsideredtobethegoldstandardforSalmonellatesting.ThePrioCHECK®SalmonellaAbporcineandthePrioCHECK®SalmonellaAbporcine2.0aretheonlyteststhatusethesameponents(antigenandreferenceserum).ThePrioCHECK®SalmonellaAbporcineELISAshaveproventheir­reliabilityinDanishcontrolprogramsandarethereforethebestchoiceinSalmonellacontrolprograms. MeanODvalue(%) LF1-1305 Convenient ThePrioCHECK®SalmonellaAbporcineELISAsfollowasimple,four-stepprotocolthatcaneasilybeautomatedandconnectedtoalaboratorymanagementsystem,allowingautomatedtrackingfromsampletoresult.Inordertofollowlong-termvariationsinhandlingofthetests,a­validationcontrolisincludedoneachplate,aswasrec­ommendedbytheOIE.ThesimpleandshortprotocolmakethePrioCHECK®Salmon­ellaAbporcineELISAsfullyautomatable. Versatile Theseversatiletestsarevalidatedforserum,plasmaandmeatjuicesamplesanddetectantibodiesagainstallserotypeswithpublichealthsignificance(theserogroupsB,C1andD).ThePrioCHECK®SalmonellaAbporcineELISAsareeconomicaltestsforsmallandlargescales­creeningofSalmonellainfectionsinpigsandareavailableinsmall(5plate)orbulk(50plate)packagesizes.ThePrioCHECK®SalmonellaAbporcineELISAscontributetofoodsafetybydetectingallimportantSalmonellatypessignificantforhumanhealth. PERFORMANCE 12010080604020 0day0 PrioCHECK®SalmonellaAbporcine2.0Competitor1Competitor2 week3 week6 Timepost-inoculation week9 ThePrioCHECK®SalmonellaAbporcine2.0identifies­experimentallyinfectedanimalsearlier.ThePrioCHECK®SalmonellaAbporcine2.0recognized25samplesaspositiveatthreeweekspost-inoculation,17ofthesesampleswereeitherpletelybyatleastpetitortest(13samples)oronlydetectedatalatertimepost-inoculation(4samples). PrioCHECK® SalmonellaAbporcineProd. No. 761048050platekit(4400tests) PrioCHECK® SalmonellaAbporcine2.0Prod. No. 76106605stripplatekit(450tests) CONTACTINFORMATION PRIONICSAGWAGISTRASSE27ACH-8952SCHLIEREN-ZURICHSWITZERLAND PHONE+41442002000FAX+41442002010INFO@PRIONICS.COMWWW.PRIONICS.COM