Screening RevolutionizingMetabolicMeasurement Promethion.25YearsofResearch. Fortwenty-fiveyears,SableSystemsInternationalhasdesignedinnovative,promisesoftwareandhardwareformetabolicmeasurementinexactingscientificapplications.Theculminationofthislegacyisourfirstproductlinedesignedspecificallyforbiomedicalresearchers,Promethion. Designedforeaseofuse,itsmodularconceptprovidesyoumaximumflexibilityintheconfigurationofyourown,uniquesystem,aswellasfutureextensibilitywhenyourresearchquestionsinevitablyevolve.Rawdatastoragemeanstherearenosecretalgorithms,orhiddenpre-conditioningofdata,justdatathatistransparentandfullytraceable.Datayoucantrust.Theparametersimportantforyourresearchcanbeextractedanywayyoulike--energyexpenditure,metabolicsubstrateselection,foodandwateruptake,mealanddrinkingpatterns,position,totalactivityandwheel-running,livebodymass–evenfullyautomatedbehavioralanalysisispossible.Allperfectlysynchronizedtothesystem’sheartbeatofonesecond.Nothingiseverlostagain. Conventionalsystemsusesealedcageswhichcausestresseffectsandrequirelonglimationtimes.ThePromethionsystemsuseconventionallive-incagesthatarenotsealed.Thisismadepossiblebyourunique,patent-pendingpull-flowgenerators.Promethion’spatent-pendingpull-flowgeneratorsallowtheuseofessentiallyanystandardlive-incagebottomformeasurement.Allyouhavetodoistoreplacethelid.Nohandlingofthemouse,nounfamiliarnewenvironment. Promethionsystemsareavailableformiceandrats.Weoffermultiplexedsystems(Promethion-M)inwhichmultiplecagesshareagasanalyzerchain,andthatoffercycletimesuptotentimesfasterthanpetition.Ifyouneedstillfastermetabolicmeasurementrates,weofferparallelcontinuoussystems(Promethion-C)inwhicheachcageispairedwithitsownflowgeneratorandgasanalyzers.Promethion-Csystemsuratelytrackmetabolicsignalscorrespondingtoevenbriefperiodsofactivity. INTEGRATEDPHENOTYPING.SYNCHRONIZED. FI-1FoodIntakeMonitorPromethionMetabolicScreeningfeatureshighprecisionsensorsformeasuringrealtimefoodintakeformiceandrats.Withupto750gramsoffoodstorageanda3milligramresolution,individualfeedingboutsandtotalfoodintakecanbemonitoredforupto30dayswithoutinterruptionorre-filling. WI-1WaterIntakeMonitorPromethionMetabolicScreeningfeatureswaterintakesensorscapableofmeasuringrealtimewaterintakeformiceandrats.Withupto500mLofwatercapacity,thewaterintakemonitormaybeuseduninterruptedforupto2weekswithoutrefilling. FAC-1essControlModulePromethionFAC-1essControlModulesputer-controlled,automatedesstofoodandwater. WM-1RunningWheelModuleThePromethionRunningWheelMonitorincorporatesadurable,stainlesssteelwheelintothecagefor180or360degreemonitoringofvoluntarywheelrevolutions.Designedtointegratewithcalorimetry,thewheelcountdatacaneasilybesynchronizedwithRQ,VO2,oranyotherparameterofthePromethionsystem. BXZ-1TotalActivityMonitorPromethionbeambreakactivitymonitorsenablerealtimeanalysisoftotalactivityandpositionwitha0.25cmcalculatedcentroid.Designedtobemodular,thePromethionbeambreakactivitymonitorcaneasilybeusedstandalone,orsynchronizedwithmetabolicmeasurement. BW-1BodyWeightModulePromethionMetabolicScreeningBodyWeightModulesarein-cageenrichmentdevicesattachedtoaPromethionuniversalMM-1loadcell.Thebodyweightmonitorallowstherealtimerecordingofbodyweightwhentheanimalinteractswiththedevice. Promethion.TheNewstandard. GasExchangeMetabolicmeasurementisacorefunctionofPromethion.Manyrefinementshaveyieldedgasanalysisinstrumentationthatisuniquelyurateandofunsurpassedresolution.Oxygenconsumptionandcarbondioxidereleaseoftheanimalaremeasured. ActivityMonitoringPromethion’sactivitydetectorsprovidepreciseinformationaboutwheelandambulatoryrunningthatcanbedirectlycorrelatedwithmetabolicdata. RQTrackchangesinsubstrateutilizationastheyhappen.Whetheranimalsrelyontheirfatreservesoractivityboutsactivatecarbohydratecatabolism-Promethionwillregisterit. FoodandWaterUptakeFoodandwateruptakearemeasureddifferentiallyanddonotsuffermonseverebaselinedriftsduetochangesincagehumidity. BodyMassInsteadofrelyingoninterpolationoverhours,seetheeffectsofeatinganddrinkingimmediately. WaterLossOnlyPromethionmeasureswaterlossrate.Forthefirsttime,waterbalancecanbeinferredinacagesetting.Thisincludesbothwaterconsumedandwatermetabolicallyproduced. Datashownisfromonecageovera20hourperiod. Promethion.VersatileMetabolicMeasurement. Calorimetry SableSystemshasspentthelast25yearsdevelopinginstrumentationthatimprovestheinvestigator’sabilitytoacquirecalorimetrydata.binesSableSystems’expertiseinanimalrespirometry,instrumentdesign,andsystemintegrationtocreateturnkeysystemsforbiomedicalresearch. IntegratedTri-GasAnalyzers Theurate,fastrespondingPromethionTri-GasAnalyzersimultaneouslymeasureswatervapor,oxygen,andcarbondioxide.Itsautomatedcorrectionsfortheeffectsofdriftandwatervapordilutionrequirenoscrubberchemicals. Intelligent,AutomatedFlowControl Optimizedforpullmoderespirometry,thePromethionpullflowcontrollerscontinuouslyorchestratemultipleflowchannelsandarecalibratedagainstNIST-Traceableflowstandards. MultiplexedorContinuousMetabolicMonitoring Promethionsystemscanbeconfiguredeitherinmultiplexedmode,sharinggasanalyzersandthusreducingoverallsystemcosts,orintrulyparallelandcontinuousmodewiththehighestlevelofmetabolicdetailpossible. Simultaneous128-CageIntegration Promethionmouseorratcagescanbeusedindividuallyorinsetsofupto128.Availablesensorsincludefoodandwatermonitors,foodesscontrol,environmentalmonitors,andactivitysensors.Allsensorsinallcagesarerecordedcontinuouslytocreatetherichestpossibledataset. SynchronousBehaviorMonitoring Wheelmonitoringworkedinfraredarraysareavailableformouseandratcagesallowingcollectionofbeambreakcounts,position,distancetraveled,andrearingdata. Promethion.CAPTUREWhatOTHERsMiss. ContinuousversusMultiplexedMetabolicMeasurement: Thechartaboveshowsthetemporalresolutionofapetitor’smultiplexedsysteminwhichseveralcagesshareagasanalysischain(redline),paredtoournewparallel,continuoussystem(blackline).Inparticularyouwillseehowournewsystemuratelytrackstheenergyexpenditurepanyingevenbriefepisodesofactivityorinactivity. Therecord-breakingtemporalresolutionofPromethion-Callowsquantificationofrapid,subtlemetabolicsignalsfrommultipleanimalsforthefirsttime.Italsoallowsformuchmoreurateallocationofenergycoststospecificbehaviorsthanhaspreviouslybeenpossible. Notallresearchneedssuchhightemporalresolution.Formanyresearchers,themoreeconomicalPromethion-Mmultiplexedsystem,whichhasuptosixfoldfastercycletimesthanthe30minutecycletimeofpetitor’ssystem(redline),willworkwell. Promethion-MMultiplexedsystems: •Sharegasanalyzersbetweencages •Patent-pending,self-calibratingpull-modegeneratorforcageairflows •Cycletimesformetabolicdataasquickas2-5minutesfor8,16or24animals •Metabolicdatacanbenon-linearlyinterpolatedbetweencycletimes,yieldingapseudo-“continuous”record •Typicaldeployment:Multiplesofeightcages •Economical Promethion-CContinuoussystems: •Eachcagehasitsown,dedicatedgasanalyzers •Patent-pending“backgroundbaselining”availablefortotallycontinuousmonitoring •Veryrapidresponsetotransienteventsduetoonesecondtemporalresolution •Allmetabolicandsensordataperfectlysynchronizedwithoutinterpolation •Typicaldeployment:Multiplesoffourcages •Z-transformcanbeappliedtometabolicdatatofurtherenhanceresponsespeed AllPromethionsystemsacquiredataeverysecondfromallsensors Ethoscan.AutomatedbehaviorAnalysis. Animal#
3 800 Non-wheelXYAmbulatoryBudget(total=286.3meters) EFOOD.10metersTFOOD.12.7metersDWATER.3.5metersTWATER.2.8metersTHOME.14.4metersLLOUNGE.67.9metersSLOUNGE.175.1meters TimeBudget.Total=21.9hours TimeBudget(total=21.9hours) Promethion’ssynchronizeddataonbodymass,foodintake,waterintake,mousemovementandwheelrevolutionsprovideprehensiveplatformforbehavioranalysis.left,averyshortsectionofadatarecordshowsamousealternatingbetweendifferentbehaviors-drinkingwater,eatingfood,enteringitshabitat(thusweighingitself)orrunningonitswheel. Totherightofthatgraph,Promethion’sEthoScanutilityextractsthetime-stampedsequenceandquantifiedlistofbehaviors(atinysectionfromamuchlongerlistisshown).Fromthislist,EthoScancancreatetimeandotionbudgetsandbehavioraltransitionmatricesforadvancedbehavioranalysis(atright). 640 480 Minutes 320 EFOODTFOODDWATERTWATERWHEELIHOMETHOMELLOUNGESLOUNGE Legend:EFOODTFOODDWATERTWATERWHEELIHOMETHOMELLNGESLNGE Interactionwithfoodhopper(significantuptakefound)Interactionwithfoodhopper(nosignificantuptakefound)Interactionwithwaterdispenser(significantuptakefound)Interactionwithwaterdispenser(nosignificantuptake)Interactionwithwheel(>=1revolution)Enteredhabitat(stablemassreading)Interactionwithhabitat(nostablemassreading)Longlounge(>60sec,nonon-XYsensorinteractions)Shortlounge(5-60sec,nonon-XYsensorinteractions) 160
0 Promethion.Additionalservices Consulting Promethion’sdataarerichenoughtoallownovelandextremelysophisticatedanalyses:mealpatternanalysis,behavioralclassificationandtimebudgeting,orcrossreferencingbehaviorandenergyexpenditureareonlysomeofthereportsthatwehavealreadyimplementedforcustomers.Atthesimplestlevel,wecanprovideanalyticalmacrosthataretailoredtoyourparticularresearchquestion,andthatextractthedatayouneedintheformatyouspecify.Atamoresophisticatedlevel,wecanpletedataanalysischainsincludingthegenerationofoverviewordetailedper-cagereports(PDFandonline)anddatavisualizationsordingtoyourrequirements.Theoutputsplywithanization’sidentitystandards(i.e.logos,colorschemes)ifyouprovidethem.And,ofcourse,youalwaysretainfullcontroloveryourdata,bothinrawandextractedform. Wedrawonthein-houseexpertiseofrealscientistswithrelevantexpertiseandpublications.Asscientiststhemselves,weareasexcitedaboutnovelinsightsasyouare. SABLECLOUD putingisaliveandwellatSable.Integratingsophisticatedanalysescannowbeassimpleasuploadingyourdata,selectingthedesiredanalyticalprotocolandreceivingtheresults.Weprovidetwomodelsofon-demanddataanalysis:anannualsubscriptionfee,orpayperuse.On-demanddataanalysiscanbinedwithconsultingtodevelopyourownproprietaryanalyticchain,whichcanthenberunondemandintheSablecloud. ComparisonofDay/NightWheelRunning.OneMouse,24hourcycle. GettingyourdatainandoutofthePromethionsystemiseasy. ExpedataFormat HDF5CSV Matlab,Mathematica,Python,
R,BigData MicrosoftExcel,otherapplications Reportslikethesecanbecreatedfromyourdataset.Justuploadyourfile,selecttheanalysisyouwanttorunandretrievethePDFreports. Promethion.IntegratedInsight. Cage1 Cage2 Cage3 Cage4 RQ RQ RQ RQ Wheelrunningspeed(m/s) Cage5 Wheelrunningspeed(m/s) Cage6 Wheelrunningspeed(m/s) Cage7 Wheelrunningspeed(m/s) Cage8 RQ RQ RQ RQ Wheelrunningspeed(m/s) Cage1 ActivitySensorswheelrunningfreerunningnotrunning Wheelrunningspeed(m/s) Cage2 Wheelrunningspeed(m/s) Cage3 Wheelrunningspeed(m/s) Cage4 Density Density Density RQ Cage5 RQ Cage6 RQ Cage7 RQ Cage8 Density Density Density Density Density RQ RQ RQ RQ EfficientWorkflow.HighThrough-put.urateResults. Promethiondelivers. TM MetabolicScreening Letushelpyouwithyourmetabolicandbehavioralmeasurementrequirements.Callustodiscussyourapplicationandobtainaquoteat (800)330-0465or(702)269-4445orfaxusat(702)269-4446Emailusatpromethion@orvisitusat ByScientists,ForScientists.